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KMID : 0356920120620020161
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Korean Journal of Anesthesiology
2012 Volume.62 No. 2 p.161 ~ p.165
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Effect of manassantin B, a lignan isolated from Saururus chinensis, on lipopolysaccharide-induced interleukin-1β in RAW 264.7 cells
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Park Hwan-Chul
Bae Hong-Beom
Jeong Cheol-Won
Lee Seong-Heon
Jeung Hye-Jin
Kwak Sang-Hyun
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Abstract
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Background: Elevated systemic levels of pro-inflammatory cytokines cause hypotension during septic shock and induce capillary leakage in acute lung injury. Manassantin B has anti-inflammatory and anti-plasmoidal properties. This study examined the effects of manassantin B on lipopolysaccharide (LPS)-induced inflammatory response in murine macrophages.
Methods: RAW 264.7 macrophage cells were incubated without or with (1, 3 and 10 μM) manassantin B and without or with (100 ng/ml) LPS. Manassantin B dissolved in phosphate buffered saline was added to the medium 1 h prior to the addition of LPS. The degree of activation of mitogen-activated protein kinase (MAPK) including extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino terminal kinases (JNK) and p38 MAPK, and the level of interleukin (IL)-1β were determined 30 min and 24 h after the addition of LPS respectively.
Results: Manassantin B inhibited the production of IL-1β and attenuated the phosphorylations of ERK1/2 and p38 MAPK, but not that of JNK, in RAW 264.7 cells treated with LPS.
Conclusions: Manassantin B reduces LPS-induced IL-1β expression through effects on ERK1/2- and p38 MAPK-mediated pathways. Manassantin B has potential as a potent anti-inflammatory drug for use in pathological processes such as sepsis or acute lung injury.
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KEYWORD
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Interleukin-1β, Lipopolysaccharides, Manassantin B, Mitogen-activated protein kinase
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